Evaluation of anti-endocrine therapy in vitro and in vivo

To evaluate the contribution of endometriotic-derived estrogens to ovarian epithelial carcinomas onset and development in vivo an experimental mouse model harbouring the two pathologic entities will be developed by inducing ovarian epithelial carcinomas in immunosuppressed mice with experimental endometriosis.The specific effects exerted by the estrogens secreted by patients-derived endometriotic tissue explants culture on cellular proliferation, migration, matrix invasion, survival and differential gene expression will be determined in human endometrioid and clear cell-derived carcinoma cell lines which express both estrogen receptors, one of them, or none. Finally, in all experimental models, the blockage of estrogens will be carried out to elucidate if this strategy has a potential therapeutic value.