NFkB pathway analysis

UNIVERSIDAD DE CHILE will study NfKB-dependent signaling both in rodent primary neurons exposed to peritoneal fluid of endometriosis patients with CHARITE, and in primary endometrial stroma cells of endometriosis patients and controls. Methods include p65-NF-B activation assays (TransAM ® kit (Active Motif Europe)) ELISAs for inflammatory NF-B-dependent cytokine expression (IL-1, IL-8), and proliferation and apoptosis assays following siRNA-mediated knockdown of components of the NFkB, pathway, including RelB.