Induced differentiation of ESCs and CSCs as a therapeutic approach

The notch and CD44 pathways will be targeted using in vitro cell culture systems after inhibition with gamma secretase inhibitors, Musashi-1 and -2 siRNA, or 4MU (see WP3) respectively, in cooperation with partners KI, WWU and CONICET. A phenotypic stem cell analysis (see WP3), apoptosis and cell viability assays, as well as sphereformation assays will be utilized as readouts.