Plan for the analysis of the functional activity of the multivirus-specific T cell product

Therefore, the functional activity of T cells specific for immunodominant epitopes derived from different proteins of the targeted viruses (e.g. CMV, EBV, and AdV) presented by different HLA alleles will be determined. On target (anti-viral) reactivity of the isolated T-cell products will be analysed upon stimulation with peptides/peptide pools of the different viral antigens, and functional reactivity will be assessed by flow cytometry using counterstaining with CD4/8 antibodies and staining for specific activation markers (CD137, CD154) and relevant cytokines (IFNγ, IL-4, GM-CSF).